Medaka opsin kinase

Rhodopsin kinase (RK) phosphorylates the light-activated rhodopsin, which increases arrestin (S-antigen) affinity to preclude further activation of transducin. RK is a member of G-protein-coupled receptor kinases (GRK). Six mammalian subtypes (GRK1-6) of GRKs have been reported so far and grouped into three subfamilies (RK, bARK and GRK4 subfamilies). The C-terminal of RK is farnesylated and anchored to the membrane.
We isolated two kinds of cDNAs (OlGRK-R and -C) encoding the putative GRKs from a medaka retinal cDNA library. It was demonstrated that OlGRK-R is localized in rods and OlGRK-C is expressed in all four types of cone cells. OlGRK-R shows a high amino acid similarity to mammalian RKs. In contrast, OlGRK-C shows less than 50 % identity with GRKs, and appears to belongs to a novel GRK subtype (GRK7) (Hisatomi et al., 1998).

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